The characteristic of the bacteria are r ed c olony (M) and w hite c olony (P). As the result, high and stable methane was produced in Rasi (I) and Amurang (III). The next step is the identification which was conducted by isolating the DNA, amplifying the DNA by PCR, then sequencing the DNA with BioEdit Sequence Aligment. Secondly, the methane produced from fermentation was analysed by g as c hromatography and the bacteria can be characterized by Bergey’s method. The samples were from four places in Minahasa, which are Rasi (I), Koka (II), Amurang (III), and Lola (IV) and one place in Bogor (V).
First, methanogenic bacteria were isolated from coconut water by fermentation. The method of this research was fermentation, analysis characterization, and identification of methanogenic bacteria. The objectives of this research are to isolate, characterize and identify the methanogenic bacteria that lived in coconut water. Most of methanogenic bacteria are uncultured, however a few of them are found in the s e wage of coconut water. Th ese bacteria produces methane, a biogas as an alternative fuel in the future. Methanogenic bacteria are one of the saprophyte bacteria. But most of the bacteria have not been identified or cultured therefore the benefits are still unknown. Some of the bacteria are saprophyte and some are parasitic. Key words: bacteria, methane, coconut waterīacteria are microbes which have an ability to live wherever there is a life. The identification of isolate M is equal with Clostridium tyrobutyricum (100 %) and isolate P is very close with Clostridium tyrobutyricum (99 %). The shape of the colonies is circles, gram positive, basil shape, mesofile, positive of catalase and citrate, positive of sugar fermentation, gelatin, casein and starch hydrolysis also lived in Nutrient Broth with pH 5.7-6.8. The characteristic of the bacteria are red colony (M) and white colony (P). Secondly, the methane produced from fermentation was analysed by gas chromatography and the bacteria can be characterized by Bergey’s method.
Most of methanogenic bacteria are uncultured, however a few of them are found in the sewage of coconut water. These bacteria produces methane, a biogas as an alternative fuel in the future. Bacteria are microbes which have an ability to live wherever there is a life.